|
| Status |
Public on Aug 26, 2014 |
| Title |
B 63N |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
AN Stage III Grade 3
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: adjacent normal
tissue: Epithelial Tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using mirVana™ miRNA isolation kit following manufacturer's instructions.
|
| Label |
Hy3
|
| Label protocol |
One µg total RNA from sample and reference was labelled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY™ LNA Array power labelling kit (Exiqon, Denmark).
|
| |
|
| Channel 2 |
| Source name |
Common reference pool
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Reference RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using mirVana™ miRNA isolation kit following manufacturer's instructions.
|
| Label |
Hy5
|
| Label protocol |
One µg total RNA from sample and reference was labelled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY™ LNA Array power labelling kit (Exiqon, Denmark).
|
| |
|
| |
| Hybridization protocol |
The Hy3™-labeled samples and a Hy5™-labeled reference RNA sample were mixed pair-wise and hybridized to the miRCURY™ LNA array version 11.0 (Exiqon, Denmark) following manufacturer's instructions usign Tecan HS4800 hybridization station (Tecan, Austria). After hybridization the slides were scanned and stored in an ozone free environment (ozon level below 2.0 ppb) in order to prevent potential bleaching of the flourescent dyes.
|
| Scan protocol |
Scanned on an Agilent G2565BA Microarray scanner.
Image analysis was carried out using the ImaGene 8.0 software (BioDiscovery,Inc.,USA).
|
| Description |
Biological Replicate 3 of 3 Adjacent Normal (AN) Stage III Grade 3
|
| Data processing |
Background corrected (Normexp with offset value 10 – Ritchie et al.,2007) and normalized using Lowess regression algorithm.
|
| |
|
| Submission date |
Aug 25, 2014 |
| Last update date |
Aug 26, 2014 |
| Contact name |
Lekha Dinesh Kumar |
| E-mail(s) |
lekha@ccmb.res.in
|
| Phone |
+91-40-27192933
|
| Organization name |
Centre for cellular and molecular biology
|
| Department |
Cancer biology
|
| Street address |
Uppal road, habshiguda
|
| City |
Hyderabad |
| State/province |
Andhra Pradesh |
| ZIP/Postal code |
500007 |
| Country |
India |
| |
|
| Platform ID |
GPL17107 |
| Series (1) |
| GSE60725 |
MicroRNA profiling of Human Breast Cancer |
|
