|
| Status |
Public on Apr 01, 2007 |
| Title |
LBN_sTX-4 |
| Sample type |
RNA |
| |
|
| Source name |
syngeneic transplanted kidney from a Lewis rat into a Lewis rat
|
| Organism |
Rattus norvegicus |
| Characteristics |
Age: 10-12 weeks
|
| Treatment protocol |
allogeneic transplanted kidney
|
| Growth protocol |
na
|
| Extracted molecule |
total RNA |
| Extraction protocol |
extraction of total RNA was performed using Qiagen Rneasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat 230 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GMS 418 Array Scanner
|
| Description |
Gene expression data from syngeneic transplanted kidney from a Lewis rat into a Lewis rat
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 11, 2006 |
| Last update date |
Dec 11, 2006 |
| Contact name |
Bayram Edemir |
| E-mail(s) |
bayram.edemir@uk-halle.de
|
| Phone |
+493455574980
|
| Organization name |
University Hospital Halle (Saale)
|
| Street address |
Ernst-Grube-Str. 20
|
| City |
Halle |
| ZIP/Postal code |
06120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE6497 |
Expression profile of syngeneic (sTX) and allogeneic kidney (aTX) transplantation compared to control (ctr) kidneys |
|
