|
| Status |
Public on Dec 01, 2014 |
| Title |
original revertant keratinocytes (P3) |
| Sample type |
RNA |
| |
|
| Source name |
original revertant keratinocytes from patient EB026-01
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: revertant keratinocytes
|
| Growth protocol |
iPSCs were cultured in the human ES cell medium. Normal FB and revertant-iPSC-FB were cultured in the 10% FBS-DMEM. Revertant-iPSC-KC and the original revertant keratinocytes were cultured in the CnT-57 (CELLnTEC).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy with on-column DNase digestion
|
| Label |
biotin
|
| Label protocol |
Extracted total RNA of each samples were reverse transcribed and amplified using the GeneChip WT PLUS Reagent Kit (WT PLUS Kit) (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cDNA was hybridized for 16 hr at 45C on GeneChip Human Transcriptome Array (HTA) 2.0 (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G3000.
|
| Description |
revertant mosacism
|
| Data processing |
The data were analyzed with Expression Console version 1.3 using Affymetrix default analysis settings and RMA as normalization method.
|
| |
|
| Submission date |
Sep 02, 2014 |
| Last update date |
Dec 01, 2014 |
| Contact name |
Jane E. Cerise |
| Organization name |
Columbia University
|
| Street address |
1150 St. Nicholas Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE60991 |
Profiling of iPSC-derived keratinocytes |
|
