|
| Status |
Public on Apr 28, 2015 |
| Title |
Spleen Cd11b+ Gr1-low myeloid suppressors from tumor bearing mice Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse spleen Cd11b+ Gr1-low cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: spleen
cell type: Cd11b+ Gr1-low cells
sample name: mds011
|
| Treatment protocol |
None
|
| Growth protocol |
Samples mds001-004, 013, 019 and 020 were derived from in vitro myeloid suppressors in IL-6 and GM-CSF according to Marigo et. Immunity 32: 790 (2010). Samples mds010 and 011 are spleen myeloid suppressors where Gr-1+ cells are depleted from the postively-sleceted CD11b+ fraction. The spleen samples originate from mice bearing EL4 flank tumors. Samples 021-023 CD14+ HLA-DR-/low were isolated from freshly obtained peripheral blood with consent from Head & Neck Squamous Cell Carcinoma patients undergoing surgical treatments at Johns Hopkins Hospital and Johns Hopkins Bayview Hospital
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen) according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from 100 ng of toal RNA according to the standard Affymetrix 3’IVT Express protocol (GeneChip 3’ IVT Express Kit 702646 Rev. 8, 2008-2010, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5-6 micrograms of biotin-labeled targets were hybridized for 16 hr at 45C on an array using the Affymetrix GeneTitan system. Following hybridization, HT_MG-430_PM and HT_HG-U133_Plus_PM plate arrays were washed and stained in the Affymetrix GeneTitan system.
|
| Scan protocol |
HT_MG-430_PM and HT_HG-U133_Plus_PM plate arrays were scanned using the Affymetrix GeneTitan system.
|
| Description |
Sample name: mds011
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console Software v1.1.
|
| |
|
| Submission date |
Sep 17, 2014 |
| Last update date |
Apr 28, 2015 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
geoffrey.neale@stjude.org
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE61479 |
Molecular control of myeloid suppressors by death pathways |
|
