|
| Status |
Public on Dec 15, 2014 |
| Title |
2133 |
| Sample type |
genomic |
| |
|
| Source name |
genomic DNA extracted and purified
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole blood
sex, 1=m, 2=f: 1
age: 68
birth-weight: 1
pair id: 71
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The blood was centrifuged at 1000 g for 10 min, and buffy-coat was frozen in aliquots at −80 °C. DNA was isolated from the buffy-coats using the salt precipitation method applying either a manual protocol or a semi-automated protocol based on the Autopure System (Qiagen, Hilden, Germany). Bisulphite treatment of 500 ng template genomic DNA was carried out with the EZ-96 DNA methylation kit (Zymo Research, Orange County, USA) following the manufacturer’s protocol.
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
MZ twin
SAMPLE 176
|
| Data processing |
minfi; Data were normalized by SWAN. Average Beta values for each sample are included in the "Processed.csv" file available on the series record.
|
| |
|
| Submission date |
Sep 17, 2014 |
| Last update date |
Dec 15, 2014 |
| Contact name |
Qihua Tan |
| E-mail(s) |
qtan@health.sdu.dk
|
| Organization name |
University of Southern Denmark
|
| Street address |
J. B. Winsløws Vej 9B
|
| City |
Denmark |
| ZIP/Postal code |
5000 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE61496 |
Epigenetic signature of birth-weight discordance in Danish twins |
|
