|
| Status |
Public on May 01, 2015 |
| Title |
Prevention-bari-2 |
| Sample type |
RNA |
| |
|
| Source name |
skin, systemic baricitinib at time of grafting, week 12
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C3H/HeJ grafted model of alopecia areata
tissue: skin
treatment: baricitinib
administered form: systemic
time of treatment: time of grafting
sampling time point: week 12
experiment type: prevention
|
| Treatment protocol |
Baricitinib was administered to mice in systemic form at the time of grafting.
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Mini Kit (Qiagen) with on-column DNase treatment (Qiagen) of skin biopsy samples taken after 12 weeks.
|
| Label |
biotin
|
| Label protocol |
NuGen Encore Biotin Protocol using 3.75 ug cDNA from NuGen Ovation RNA Amplification V2 system.
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.75 ug of cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G3000.
|
| Description |
Bari2
|
| Data processing |
The data were analyzed with Bioconductor/R and were RMA normalized.
|
| |
|
| Submission date |
Sep 18, 2014 |
| Last update date |
May 01, 2015 |
| Contact name |
Ali Jabbari |
| Organization name |
University of Iowa
|
| Street address |
200 Hawkins Dr, 40025 PFP
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE61551 |
Treatment of C3H/HeJ grafted mice with baricitinib [prevention] |
| GSE61555 |
Treatment of C3H/HeJ grafted mice with baricitinib |
|
