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Sample GSM1508104 Query DataSets for GSM1508104
Status Public on May 01, 2015
Title Topical-bari-w0-1
Sample type RNA
 
Source name skin, topical baricitinib after disease establishment, week 0
Organism Mus musculus
Characteristics strain/background: C3H/HeJ grafted model of alopecia areata
tissue: skin
treatment: baricitinib
administered form: topical
time of treatment: after disease establishment
sampling time point: week 0
experiment type: topical treatment
Treatment protocol Baricitinib was administered to mice in topical form after the establishment of disease.
Growth protocol n/a
Extracted molecule total RNA
Extraction protocol miRNeasy Mini Kit (Qiagen) with on-column DNase treatment (Qiagen) of skin biopsy samples taken at the start of treatment or after 12 weeks.
Label biotin
Label protocol NuGen Encore Biotin Protocol using 3.75 ug cDNA from NuGen Ovation RNA Amplification V2 system.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G3000.
Description B0_371
Data processing The data were analyzed with Bioconductor/R and were RMA normalized.
 
Submission date Sep 18, 2014
Last update date May 01, 2015
Contact name Ali Jabbari
Organization name University of Iowa
Street address 200 Hawkins Dr, 40025 PFP
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platform ID GPL1261
Series (2)
GSE61554 Treatment of C3H/HeJ grafted mice with baricitinib [topical]
GSE61555 Treatment of C3H/HeJ grafted mice with baricitinib

Data table header descriptions
ID_REF
VALUE RMA normalized log2 expression value

Data table
ID_REF VALUE
1415670_at 7.974784304
1415671_at 9.903931746
1415672_at 10.22292265
1415673_at 8.183162313
1415674_a_at 8.801826482
1415675_at 8.900623727
1415676_a_at 9.927009493
1415677_at 8.968172205
1415678_at 11.92083941
1415679_at 10.79128296
1415680_at 8.452487426
1415681_at 9.319582205
1415682_at 8.555501329
1415683_at 11.04351213
1415684_at 8.604587147
1415685_at 7.815950681
1415686_at 11.07124293
1415687_a_at 11.6465055
1415688_at 10.51920836
1415689_s_at 8.093785069

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM1508104_B0_371.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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