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Sample GSM1508112 Query DataSets for GSM1508112
Status Public on May 01, 2015
Title Topical-control-w0-1
Sample type RNA
 
Source name skin, vehicle control after disease establishment, week 0
Organism Mus musculus
Characteristics strain/background: C3H/HeJ grafted model of alopecia areata
tissue: skin
treatment: vehiclecontrol
administered form: topical
time of treatment: after disease establishment
sampling time point: week 0
experiment type: topical treatment
Treatment protocol Baricitinib was administered to mice in topical form after the establishment of disease.
Growth protocol n/a
Extracted molecule total RNA
Extraction protocol miRNeasy Mini Kit (Qiagen) with on-column DNase treatment (Qiagen) of skin biopsy samples taken at the start of treatment or after 12 weeks.
Label biotin
Label protocol NuGen Encore Biotin Protocol using 3.75 ug cDNA from NuGen Ovation RNA Amplification V2 system.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G3000.
Description C0_359
Data processing The data were analyzed with Bioconductor/R and were RMA normalized.
 
Submission date Sep 18, 2014
Last update date May 01, 2015
Contact name Ali Jabbari
Organization name University of Iowa
Street address 200 Hawkins Dr, 40025 PFP
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platform ID GPL1261
Series (2)
GSE61554 Treatment of C3H/HeJ grafted mice with baricitinib [topical]
GSE61555 Treatment of C3H/HeJ grafted mice with baricitinib

Data table header descriptions
ID_REF
VALUE RMA normalized log2 expression value

Data table
ID_REF VALUE
1415670_at 8.708476866
1415671_at 10.46144567
1415672_at 10.03750532
1415673_at 9.447247616
1415674_a_at 9.300167544
1415675_at 9.190523872
1415676_a_at 11.65811073
1415677_at 10.03023404
1415678_at 12.11564117
1415679_at 10.91667403
1415680_at 9.137329304
1415681_at 9.750814928
1415682_at 8.556359663
1415683_at 10.8456137
1415684_at 8.382506308
1415685_at 7.700308801
1415686_at 11.25641364
1415687_a_at 12.15590833
1415688_at 10.4278649
1415689_s_at 8.098086405

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM1508112_C0_359.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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