|
| Status |
Public on Mar 01, 2007 |
| Title |
KIU_101B88 (HG-U133B) |
| Sample type |
RNA |
| |
|
| Source name |
breast
|
| Organism |
Homo sapiens |
| Characteristics |
samplename: KIU_101B88
ID: 101B88
series: KIU
age: 40
grade: 3
size: 1.2
er: 0
pgr: 0
node: 0
t.rfs: 2280
e.rfs: 0
t.dmfs: 2280
e.dmfs: 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)
|
| Label |
biotin
|
| Label protocol |
Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Tumor samples from untreated primary breast cancer patients.
|
| Data processing |
RMA Bioconductor
|
| |
|
| Submission date |
Dec 14, 2006 |
| Last update date |
Apr 14, 2011 |
| Contact name |
Benjamin Haibe-Kains |
| E-mail(s) |
benjamin.haibe.kains@utoronto.ca
|
| Phone |
+14165818626
|
| Organization name |
Princess Margaret Cancer Centre
|
| Department |
Princess Margaret Research
|
| Lab |
Bioinformatics and Computational Genomics
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE6532 |
Definition of clinically distinct molecular subtypes in estrogen receptor positive breast carcinomas using genomic grade |
|
