|
| Status |
Public on Nov 03, 2014 |
| Title |
T215P_eh0026 |
| Sample type |
RNA |
| |
|
| Source name |
BALB/c_PR8 NS1 T215P variant_lung
|
| Organism |
Mus musculus |
| Characteristics |
strain background: BALB/c
infected with: Influenza A virus PR8 NS1 T215P variant
time point: 5 dats after infection
tissue: lung
|
| Growth protocol |
BALB/c mice were infected with Influenza A virus PR8 wt and PR8 NS1 T215P variant (100PFU). PBS inoculated mice served as control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
On d5 after Infection, lungs were collected in Trizol. RNA isolation was done using Trizol method and PureLink RNA Mini kit (Ambion)
|
| Label |
Biotin
|
| Label protocol |
RNA was amplified using the NuGEN Ovation Pico WTA V2 system and then labeled with the NuGEN Encore Biotin Module.
|
| |
|
| Hybridization protocol |
Labeled targets were hybridized on the mogene2st array and processed using the Affymetrix system.
|
| Scan protocol |
Scanning uses the Affymetrix scanner
|
| Data processing |
The data were analyzed with Partek Genomics Suite 6.6 by the RMA method.
|
| |
|
| Submission date |
Oct 08, 2014 |
| Last update date |
Nov 04, 2014 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE62169 |
NS1-mediated inhibition of c-Abl results in acute lung injury and priming for bacterial co-infections; insights into 1918 H1N1 pandemic |
|
