|
| Status |
Public on Oct 01, 2015 |
| Title |
Striatal microglia at 4month, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Microglia extracted from striatum at 4 month of age
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain
genotype: C57BL/6J
age: 4month
|
| Growth protocol |
C57BL/6J mice maintained under standard conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Plus Micro Kit (isolated microglia) and Rneasy Midi Kit (regional homogenates) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 6 ug of cRNA were hybridized for 16 hr at 45C to the Affymetrix HT_MG-430_PM-16 plate array in the Gene Titan using the Affymetrix standard protocols.
|
| Scan protocol |
GeneChips were scanned using the Gene Titan Multi-channel instrument from Affymetrix.
|
| Description |
Gene expression data from striatal microglia of 4 month old mice
|
| Data processing |
The data were analyzed with Affymetric Expression Console built-in Robust Multiarray Averaging (RMA) method using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Oct 16, 2014 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Barry W McColl |
| E-mail(s) |
barry.mccoll@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
Centre for Discovery Brain Sciences
|
| Street address |
49 Little France Crescent
|
| City |
Edinburgh |
| ZIP/Postal code |
EH16 4SB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE62420 |
The regional mouse microglial transcriptome during aging |
|
