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Sample GSM1527315 Query DataSets for GSM1527315
Status Public on Mar 18, 2015
Title DOHH2-ST-7612-3
Sample type RNA
 
Source name DOHH2 cell lines treated with ST7612AA1
Organism Homo sapiens
Characteristics cell line: DOHH2
cell type: lymphoma cell line
treated with: ST7612AA1
Treatment protocol treatment with pan-histone deacetylase inhibitor (HDACi), a thioacetate-ω (γ-lactam amide) derivative.
Growth protocol in vitro
Extracted molecule total RNA
Extraction protocol RNA extraction was performed using a commercially available kit (RNeasy, Qiagen, Frederick,MD) according to the manufactures’ recommendations. RNA quality was confirmed based on a RNA integrity number >8 by use of an electrophoresis bioanalyzer (2100 Agilent Bioanalyser).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification Kit for Illumina arrays
 
Hybridization protocol Whole Genome Gene Expression Direct Hybridization
Scan protocol Illumina HiScan
Description 9287794016_F
Data processing Signal calculated by GenomeStudio Data Analysis Software’s Gene Expression Module (GSGX) Version 1.5.4 (Illumina, Inc.)
The data were normalised using quantile method
 
Submission date Oct 17, 2014
Last update date Mar 18, 2015
Contact name Francesco Bertoni
E-mail(s) francesco.bertoni@ior.usi.ch
Phone +41 91 820 0367
Organization name IOR
Department Lymphoma & Genomics Research Program
Street address Via V. Vela 6
City Bellinzona
State/province Ticino
ZIP/Postal code 6500
Country Switzerland
 
Platform ID GPL10558
Series (1)
GSE62460 Preclinical antitumor activity of ST7612AA1: a novel second generation oral histone deacetylase (HDAC) inhibitor

Data table header descriptions
ID_REF
VALUE Log2 Normalized Data

Data table
ID_REF VALUE
ILMN_1762337 6.596527029
ILMN_2055271 6.809903922
ILMN_1736007 6.624182004
ILMN_2383229 6.598427399
ILMN_1806310 6.688174239
ILMN_1779670 6.577774148
ILMN_1653355 6.665036397
ILMN_1717783 6.480126569
ILMN_1705025 6.52799654
ILMN_1814316 6.620892032
ILMN_2359168 6.528726069
ILMN_1731507 6.541420435
ILMN_1787689 6.557817421
ILMN_3241953 8.25471192
ILMN_1745607 6.532261417
ILMN_2136495 6.528958767
ILMN_1668111 6.540583168
ILMN_2295559 6.57168577
ILMN_1735045 6.848167398
ILMN_1680754 6.678569471

Total number of rows: 47231

Table truncated, full table size 1148 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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