|
| Status |
Public on Oct 22, 2014 |
| Title |
CD19 Relapse (2992ONBC82) |
| Sample type |
RNA |
| |
|
| Source name |
CD19+ blood cells from ON patient in relapse
|
| Organism |
Homo sapiens |
| Characteristics |
age (years): 45
gender: female
disease state: ON patient in relapse
cell type: CD19+ blood cells
|
| Treatment protocol |
PBMC were purified using Ficoll hypaque gradient (Invitrogen, USA). Cell subsets of CD4+, CD8+ T lymphocytes , CD19+ B cells and CD14+ macrophages sub-populations were isolated using FITC-conjugated magnetic microbeads (Pharmigen, USA)(purity >95%) Microarray gene expression was performed separately for each cell sub-population.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression of CD19+ blood cells from ON patient in relapse
|
| Data processing |
Summarized/scaled/normalized probeset data generated by GC-RMA
|
| |
|
| Submission date |
Oct 22, 2014 |
| Last update date |
Oct 22, 2014 |
| Contact name |
Michael Gurevich |
| E-mail(s) |
gurevich@sheba.health.gov.il
|
| Organization name |
Sheba Medical Center
|
| Department |
Multiple Sclerosis Center
|
| Lab |
Neurogenomics
|
| Street address |
Tel-Hashomer
|
| City |
Ramat-Gan |
| ZIP/Postal code |
52621 |
| Country |
Israel |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE62584 |
PBMC gene expression of early onset of the first demyelinating event of acute optic neuritis |
|
