|
| Status |
Public on Oct 23, 2014 |
| Title |
M-/-.WT I UR |
| Sample type |
RNA |
| |
|
| Source name |
CSF-1 starved, M-/-.WT cells
|
| Organism |
Mus musculus |
| Characteristics |
treatment: CSF-1 starved
cell line: M-/-.WT cells
|
| Treatment protocol |
Cells were either starved overnight for CSF-1 and subsequently stimulated with CSF-1 for 0min, 20 min, 60 min and 180 min, or constitutively grown in CSF-1.
|
| Growth protocol |
M-/-.WT, M-/-.Y721F, M-/-.3ABY721, M-/-.3AB cell lines were cultured in alpha modified Eagle’s medium (α-MEM) containing 10% newborn calf serum, 100 U/ml penicillin, 100 µg/ml streptomycin. Unless otherwise specified 120 ng/mL human recombinant CSF-1 was added to the cultures.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared by the Genomics core at Einstein according to the standard Affymetrix protocol from 100 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized by the Genomics Core at Einstein, according to manufacturer’s instructions, for 16 hr at 45C on GeneChip Mouse Gene Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned by the Genomics Core at Einstein using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
GeneChip Mouse Gene 1.0 ST Array
|
| Data processing |
R/Bioconductor
|
| |
|
| Submission date |
Oct 22, 2014 |
| Last update date |
Oct 23, 2014 |
| Contact name |
E. Richard Stanley |
| E-mail(s) |
richard.stanley@einstein.yu.edu
|
| Phone |
7184302344
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Dev and Molecular Biology
|
| Lab |
Chanin 507
|
| Street address |
1300 Morris Park Ave
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE62630 |
Colony stimulating factor-1 receptor signaling networks inhibit macrophage inflammatory responses by induction of microRNA-21 |
|
