|
| Status |
Public on Nov 05, 2014 |
| Title |
Jag1+/-Rfng+/-_portal tract Rep 5 |
| Sample type |
RNA |
| |
|
| Source name |
1 week old mut
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Jag1+/-Rfng+/-
age: 1 week
tissue: portal tract
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 12-20 portal tracts per replicate from frozen sections using the PicoPure RNA isolation kit. Samples were Dnased during purification protocol.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared using 5ug of template according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Folllowing fragmentation, 3.4ug of labeled products were hybridized for 16hr at 45C on GeneChip Mouse Gene 2.0 ST Arrays.Chips were washed and stained according to Affymetrix guidelines.
|
| Scan protocol |
GeneChips were scanned using a GeneChip 3000 7G scanner.
|
| Description |
Sample name: dbl_2304-2 excl
portal tracts were laser capture-microdissected
|
| Data processing |
Raw data was converted to average intensities using Affymetrix Command Console software. Robust Multi-array Average normalization was applied using Partek Genomics Suite software (v6.6).Significance Analysis of Microarrays was applied yielding q-values and fold changes to identify differentially expressed genes .
|
| |
|
| Submission date |
Nov 05, 2014 |
| Last update date |
Nov 05, 2014 |
| Contact name |
Matthew Joseph Ryan |
| E-mail(s) |
ryanma@email.chop.edu
|
| Phone |
267-426-7223
|
| Organization name |
The Children's Hospital of Philadelphia
|
| Department |
Pediatrics
|
| Street address |
34th and Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE62991 |
Liver expression data from 1 week old mouse portal tracts |
|
