|
| Status |
Public on Nov 12, 2014 |
| Title |
Melanoma cell line M010817, siControl transfected, biol Rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Melanoma cell line transfected for 72h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: M010817
transfection: siControl
tissue: Subcutaneous melanoma metastasis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
According to RNeasy Mini Kit (74104, Qiagen)
|
| Label |
biotin
|
| Label protocol |
According to MessageAmp II-Biotin Enhanced aRNA Amplification Kit (AM1791, Life Technologies)
|
| |
|
| Hybridization protocol |
According to Human Gene 2.1 ST Array (902136, Affymetrix) protocol and GeneChip Fluidics Station 450 (Affymetrix)
|
| Scan protocol |
Scanned with a GeneChip Scanner 7G (Affymetrix)
|
| Data processing |
Differential gene expression was determined by R package limma (Smyth, G. K. limma: Linear Models for Microarray Data. Statistics for Biology and Health 5, 397–420 (2005))
|
| |
|
| Submission date |
Nov 10, 2014 |
| Last update date |
Nov 12, 2014 |
| Contact name |
Daniel Zingg |
| E-mail(s) |
daniel.zingg@uzh.ch
|
| Organization name |
University of Zurich
|
| Department |
Institute of Anatomy
|
| Lab |
Lukas Sommer Lab
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL17692 |
| Series (1) |
| GSE63165 |
The epigenetic modifier EZH2 controls melanoma growth and metastasis through silencing of distinct tumour suppressors |
|
