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Sample GSM1563651 Query DataSets for GSM1563651
Status Public on Dec 12, 2014
Title A01068_q3
Sample type RNA
 
Channel 1
Source name Mycobacterium tuberculosis CDC1551 D-Rv2745c knockout mutant
Organism Mycobacterium tuberculosis CDC1551
Characteristics genotype/variation: D-Rv2745c knockout mutant
time point: t=0
treatment: hypoxia
Treatment protocol Bacterial cultures were treated with either hypoxia or reaeration
Growth protocol Mtb strains were cultured as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
Extracted molecule total RNA
Extraction protocol RNA was extracted as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
Label Cy3
Label protocol cDNA labeling was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
 
Channel 2
Source name Mycobacterium tuberculosis CDC1551 D-Rv2745c knockout mutant
Organism Mycobacterium tuberculosis CDC1551
Characteristics genotype/variation: D-Rv2745c knockout mutant
time point: t = 6 hr
treatment: reaeration
Treatment protocol Bacterial cultures were treated with either hypoxia or reaeration
Growth protocol Mtb strains were cultured as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
Extracted molecule total RNA
Extraction protocol RNA was extracted as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
Label Cy5
Label protocol cDNA labeling was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
 
 
Hybridization protocol Hybridization to arrays was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
Scan protocol Slides were scanned as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
Data processing Data analysis was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
 
Submission date Dec 11, 2014
Last update date Dec 12, 2014
Contact name Deepak Kaushal
E-mail(s) dkaushal@tulane.edu
Organization name Tulane National Primate Research Center
Department Bacteriology
Lab Kaushal
Street address 18703 Three Rivers Rd
City Covington
State/province LA
ZIP/Postal code 38105
Country USA
 
Platform ID GPL18320
Series (1)
GSE64065 The Mycobacterium tuberculosis Clp Gene Regulator is Required for in vitro Reactivation from Hypoxia-induced Dormancy

Data table header descriptions
ID_REF
VALUE Values are expression of M, i.e. a log2 ratio of treated sample at a particular time in a particular strain to the pre-treatment sample from the same strain, e.g Mtb treated with either hypoxia or reaeration relative to Mtb prior to hypoxia treatment

Data table
ID_REF VALUE
MT0001 -1.023873715
MT0002 0.524658525
MT0003 0.70018563
MT0004 0
MT0005 -1.023247814
MT0006 -1.612457606
MT0007
MT0009 0.629834664
MT0010 0.270235485
MT0011 1.732996374
MT0012 0
MT0013 -0.885206337
MT0014 0
MT0015 2.624087154
MT0016 0
MT0017 -1.784252306
MT0018 0
MT0019 -3.251817436
MT0020 0
MT0021 1.969468506

Total number of rows: 4144

Table truncated, full table size 56 Kbytes.




Supplementary file Size Download File type/resource
GSM1563651_A01068_q3.gpr.gz 1.7 Mb (ftp)(http) GPR
Processed data included within Sample table

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