|
Status |
Public on Dec 12, 2014 |
Title |
A01068_q3 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Mycobacterium tuberculosis CDC1551 D-Rv2745c knockout mutant
|
Organism |
Mycobacterium tuberculosis CDC1551 |
Characteristics |
genotype/variation: D-Rv2745c knockout mutant time point: t=0 treatment: hypoxia
|
Treatment protocol |
Bacterial cultures were treated with either hypoxia or reaeration
|
Growth protocol |
Mtb strains were cultured as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
Label |
Cy3
|
Label protocol |
cDNA labeling was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
|
|
Channel 2 |
Source name |
Mycobacterium tuberculosis CDC1551 D-Rv2745c knockout mutant
|
Organism |
Mycobacterium tuberculosis CDC1551 |
Characteristics |
genotype/variation: D-Rv2745c knockout mutant time point: t = 6 hr treatment: reaeration
|
Treatment protocol |
Bacterial cultures were treated with either hypoxia or reaeration
|
Growth protocol |
Mtb strains were cultured as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
Label |
Cy5
|
Label protocol |
cDNA labeling was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
|
|
|
Hybridization protocol |
Hybridization to arrays was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
Scan protocol |
Slides were scanned as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
Data processing |
Data analysis was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
|
|
Submission date |
Dec 11, 2014 |
Last update date |
Dec 12, 2014 |
Contact name |
Deepak Kaushal |
E-mail(s) |
dkaushal@tulane.edu
|
Organization name |
Tulane National Primate Research Center
|
Department |
Bacteriology
|
Lab |
Kaushal
|
Street address |
18703 Three Rivers Rd
|
City |
Covington |
State/province |
LA |
ZIP/Postal code |
38105 |
Country |
USA |
|
|
Platform ID |
GPL18320 |
Series (1) |
GSE64065 |
The Mycobacterium tuberculosis Clp Gene Regulator is Required for in vitro Reactivation from Hypoxia-induced Dormancy |
|