|
| Status |
Public on Dec 12, 2014 |
| Title |
355219_q3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mycobacterium tuberculosis CDC1551 D-Rv2745c knockout mutant
|
| Organism |
Mycobacterium tuberculosis CDC1551 |
| Characteristics |
genotype/variation: D-Rv2745c knockout mutant
time point: t=0
treatment: hypoxia
|
| Treatment protocol |
Bacterial cultures were treated with either hypoxia or reaeration
|
| Growth protocol |
Mtb strains were cultured as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
| Label |
Cy3
|
| Label protocol |
cDNA labeling was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
| |
|
| Channel 2 |
| Source name |
Mycobacterium tuberculosis CDC1551 D-Rv2745c knockout mutant
|
| Organism |
Mycobacterium tuberculosis CDC1551 |
| Characteristics |
genotype/variation: D-Rv2745c knockout mutant
time point: t = 48 hr
treatment: reaeration
|
| Treatment protocol |
Bacterial cultures were treated with either hypoxia or reaeration
|
| Growth protocol |
Mtb strains were cultured as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
| Label |
Cy5
|
| Label protocol |
cDNA labeling was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
| |
|
| |
| Hybridization protocol |
Hybridization to arrays was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
| Scan protocol |
Slides were scanned as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
| Data processing |
Data analysis was performed as described earlier (Mehra and Kaushal, PMCID: PMC2698404)
|
| |
|
| Submission date |
Dec 11, 2014 |
| Last update date |
Dec 12, 2014 |
| Contact name |
Deepak Kaushal |
| E-mail(s) |
dkaushal@tulane.edu
|
| Organization name |
Tulane National Primate Research Center
|
| Department |
Bacteriology
|
| Lab |
Kaushal
|
| Street address |
18703 Three Rivers Rd
|
| City |
Covington |
| State/province |
LA |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL18320 |
| Series (1) |
| GSE64065 |
The Mycobacterium tuberculosis Clp Gene Regulator is Required for in vitro Reactivation from Hypoxia-induced Dormancy |
|
