|
| Status |
Public on Dec 15, 2014 |
| Title |
hippocampus_freshfrozen_43 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampal formation
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: hippocampal formation
phenotype: epilepsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from hippocampal tissue samples using ALLPrep DNA/RNA Mini Kit (Qiagen, Hilden) according manufacturer's protocoll. Tissue lysate was passed trough an AllPrep Rneasy spin collum to selectively isolate RNA.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with Illumina TotalPrep-96 RNA Amplification Kit (Life Technologies Corporation, Darmstadt).
|
| |
|
| Hybridization protocol |
Illumina Direct Hybridization Assay Kit according standard protocol
|
| Scan protocol |
Standard Illumina scanning protocol uisng Illumina BeadArray Reader
|
| Description |
human brain tissue from pharmacoresistant TLE patients
|
| Data processing |
Gene expression data were normalized using Illumina BeadStudio software suite by means of quantile normalization with background subtraction
|
| |
|
| Submission date |
Dec 15, 2014 |
| Last update date |
Dec 15, 2014 |
| Contact name |
Albert Becker |
| Organization name |
University of Bonn Medical Center
|
| Department |
Neuropathology
|
| Street address |
Sigmund-Freud Str. 25
|
| City |
Bonn |
| ZIP/Postal code |
53127 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE63808 |
chronic temporal lobe epilepsy: biopsy hippocampus |
|
