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Sample GSM1566666 Query DataSets for GSM1566666
Status Public on Nov 25, 2021
Title sirt3ko_control biological replicate 1
Sample type RNA
 
Source name Sirt3 knockout mouse, control diet
Organism Mus musculus
Characteristics genotype/variation: Sirt3 knockout
strain: C57BL/6
gender: male
diet: control
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix Mouse Gene 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
Data processing The data were analyzed with Affymetrix Exression Console version 1.4 using RMA
 
Submission date Dec 16, 2014
Last update date Nov 25, 2021
Contact name Jamie Barger
E-mail(s) jamie.l.barger@gmail.com
Organization name LifeGen Technologies, LLC
Street address 510 Charmany Drive, Suite 263
City Madison
State/province WI
ZIP/Postal code 53719
Country USA
 
Platform ID GPL6246
Series (1)
GSE64229 Gene expression in gastrocnemius muscle in response to calorie restriction in wildtype and Sirt3 knockout mice

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
10338001 11.8489
10338002 5.94414
10338003 10.1647
10338004 9.06151
10338005 1.64051
10338006 1.89369
10338007 2.32894
10338008 3.41605
10338009 7.81485
10338010 1.67796
10338011 5.45973
10338012 1.75638
10338013 1.5464
10338014 1.58848
10338015 1.55058
10338016 7.13915
10338017 12.7233
10338018 6.36968
10338019 4.8942
10338020 7.62208

Total number of rows: 35556

Table truncated, full table size 586 Kbytes.




Supplementary file Size Download File type/resource
GSM1566666_Sirt3ko_co06.CEL.gz 3.8 Mb (ftp)(http) CEL
GSM1566666_Sirt3ko_co06.rma-gene-default.chp.gz 269.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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