|
| Status |
Public on Jan 30, 2015 |
| Title |
Androgen Sensitive Met (matched) (RS-01574312) |
| Sample type |
SRA |
| |
|
| Source name |
Orthotopic Tumor
|
| Organism |
Mus musculus |
| Characteristics |
tumor type: metastatic
phenotype: androgen sensitive
|
| Growth protocol |
Myc-CaP AS or CR cell lines were injected into anterior prostate lobes of FVB male mice eith intact or surgically precastrated. Resulting primary and met tumors were utilized for RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Basecalls performed using CASAVA version 1.8.2
Sequences reads were trimmed with adaptor sequence and mapped to ensembl GRCm38 reference with anntation GTF version 71 using tophat 2.0
Reads counts are calculated by R scripts with Rsamtools and GenomicRanges package.
Genome_build: GRCm38
Supplementary_files_format_and_content: tab-delimited text files include counts for each Sample.
|
| |
|
| Submission date |
Jan 07, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Qiang Hu |
| E-mail(s) |
Qiang.Hu@RoswellPark.org
|
| Organization name |
Roswell Park Cancer Institute
|
| Street address |
Elm & Carlton Streets
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14263 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE64771 |
Top2a identifies and provides epigenetic rationale for novel combination therapeutic strategies for aggressive prostate cancer |
|
| Relations |
| BioSample |
SAMN03278292 |
| SRA |
SRX833876 |
