All animals were bred at UNC from mice that were less than six generations removed from founders acquired from the Jackson Laboratory (Bar Harbor, ME).
Growth protocol
NA
Extracted molecule
total RNA
Extraction protocol
Tissues were rapidly dissected, snap-frozen in liquid nitrogen, and pluverized using a BioPulverizer unit (BioSpec Products, Bartlesville, OK)
Label
biotin
Label protocol
NA
Hybridization protocol
Total RNA were hybridized to Affymetrix Mouse Gene 1.1 ST 96-Array Plate arrays using a GeneTitan instrument from Affymetrix according to the manufacturer’s protocols
Scan protocol
NA
Description
gene expression data
Data processing
Robust multiarray average method (RMA) implemented in the Affymetrix gene expression console with default settings (median polish and sketch-quantile normalization) to estimate normalized expression levels of transcripts
