|
| Status |
Public on Apr 13, 2018 |
| Title |
TAA_F_20ppm_15d_1 |
| Sample type |
RNA |
| |
|
| Source name |
Thioacetamid, female, 14 days
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1
gender: female
tissue: liver
treatment short name: TAA
treatment: Thioacetamid
treatment duration: 14 days
treatment dose: 2 mg/kg/d
treatment group: TAA_F_20ppm_15d
corresponding control: CMC_F_G41_15d
|
| Treatment protocol |
After 3 and 14 days of compound treatment mice were anaesthetized in isoflurane and exsanguinated from the heart and subjected to necropsy. The left lateral liver lobe was extracted and snap frozen under -80°C conditions.
|
| Growth protocol |
CD-1 male and female mice were orally treated with a set of genotoxic carcinogens (CIDB, DMN and MDA), nongenotoxic carcinogens (DCB, PB and PBO), non-hepatocarcinogens (CFX, Praz and Prop) and a group of undefined compounds (CPA, Wy-14643 and TAA) over a period of 3 and 14 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Liver tissue was homogenized and RNA isolation was performed with Qiazol extraction (Qiagen, Hilden,Germany). RNA integrity was checked using the Agilent Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
100 ng Total RNA (diluted to 50ng/µl) were labelled using miRNA complete labelling (PCp-Cy3) and hybridization kit (Agilent# 5190-0456).
|
| |
|
| Hybridization protocol |
Agilent Mouse miRNA arrays (G4472A) were hybridized, washed and scanned on an Agilent Microarray Scanner following manufacturer instructions.
|
| Scan protocol |
Arrays ware scanned on an Agilent Microarray Scanner. Images from the scanner were processed using Agilent Feature Extraction Software v10.5.1.
|
| Data processing |
The raw microarray data was normalized using the RMA method implemented in R/Bioconductor.
|
| |
|
| Submission date |
Apr 15, 2015 |
| Last update date |
Apr 13, 2018 |
| Contact name |
Jonathan Moggs |
| E-mail(s) |
jonathan.moggs@novartis.com
|
| Organization name |
Novartis
|
| Street address |
Fabrikstrasse 2
|
| City |
Basel |
| ZIP/Postal code |
4056 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL13493 |
| Series (2) |
| GSE67943 |
Transcriptional profiling of microRNAs after short-term exposure of CD1-mice to carcinogenic and non-carcinogenic chemicals |
| GSE68387 |
IMI MARCAR Project: towards novel biomarkers for cancer risk assessment |
|
