|
| Status |
Public on Jul 15, 2015 |
| Title |
B2_GlomAffyAnnot_Other_44 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney Biopsy from NEPTUNE subject
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: kidney
compartment: glomerulus
histopath: Other
apol1 risk status: High-Risk
patient age: 14
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Rneasy kit (Qiagen) following manufacturer's protocol
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
not provided
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene expression from glomerulus
Batch 1 and 2 normalizations are available on the series record. The batch-corrected normalized data are available in the sample tables.
|
| Data processing |
Preprocessing, normalization with RMA, background correction performed with affymetrix powertools
|
| |
|
| Submission date |
Apr 21, 2015 |
| Last update date |
Feb 01, 2017 |
| Contact name |
Matthew Gordon Sampson |
| E-mail(s) |
mgsamps@med.umich.edu
|
| Organization name |
University of Michigan School of Medicine
|
| Department |
Pediatrics
|
| Street address |
8220DMSRB3 1150 West Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17692 |
| Series (2) |
| GSE68125 |
Integrative genomics identifies novel associations with APOL1 risk genotype in African American NEPTUNE subjects [glomerulus] |
| GSE68127 |
Integrative genomics identifies novel associations with APOL1 risk genotype in African American NEPTUNE subjects |
|
