|
| Status |
Public on Dec 14, 2015 |
| Title |
Diabetic Nephropathy Cprobe_17 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney Biopsy from Human Diabetic Nephropathy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Tubulointerstitium from kidney biopsy
|
| Treatment protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Rneasy kit (Qiagen) following manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene Expression data from the tubulointerstitial compartment of human Diabetic Nephropathy kidney
|
| Data processing |
The data were analyzed using GenePattern pipeline: RMA method and Human Entrez Gene custom CDF annotation version 10 was used for the normalization
Each of the hybridization was normalized seperately and the last sheet contains the batchcorrected data using Combat
|
| |
|
| Submission date |
Jun 01, 2015 |
| Last update date |
Dec 14, 2015 |
| Contact name |
Viji Nair |
| E-mail(s) |
vijin@med.umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1152 West Medical Centre Dr.
|
| City |
Ann Arbor |
| ZIP/Postal code |
48331 |
| Country |
USA |
| |
|
| Platform ID |
GPL11670 |
| Series (1) |
| GSE69438 |
Tissue Transcriptome Driven Identification of Epidermal Growth Factor as a Chronic Kidney Disease Biomarker |
|
