|
| Status |
Public on Feb 03, 2017 |
| Title |
siP/CAF 1 |
| Sample type |
RNA |
| |
|
| Source name |
Rh30 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Rhabdomyosarcoma Cell Line
cell line: Rh30
treatment: siP/CAF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep RNA Amplification kit for Illumina array (HT12)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 4_siP/CAF 1
|
| Data processing |
The data were normalised using quantile normalisation with GeneSpring GX v 10.0.2. Quantile normalization was performed for each sample individually and baseline was corrected to each probe across the samples in the experiment.
|
| |
|
| Submission date |
Jun 01, 2015 |
| Last update date |
Feb 03, 2017 |
| Contact name |
madavan vasudevan |
| E-mail(s) |
madavan.vasudevan@gmail.com
|
| Phone |
+91-9845093355
|
| Organization name |
Theomics International Private Limited
|
| Street address |
Kasturi Nagar
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560043 |
| Country |
India |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE69443 |
P/CAF mediates Pax3-FKHR dependent oncogenesis in alveolar rhabdomyosarcoma |
|
