|
| Status |
Public on Dec 09, 2016 |
| Title |
A_135 |
| Sample type |
RNA |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
cohort: Healthy, non-smoking
gender: male
race: white_caucasian
tissue: blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from blood collected in PAXgene tubes using the PAXgene blood miRNA kit
|
| Label |
biotin
|
| Label protocol |
Total RNA was reverse-transcribed and amplified using the NuGen Ovation™ RNA amplification system V2 with Ovation™WB reagent. Amplified RNA was fragmented and labeled using the NuGen FL-Ovation™ cDNA Biotin Module V2. Amplification and labeling were performed on a Beckman-Coulter ArrayPlex.
|
| |
|
| Hybridization protocol |
Standard procedure for Affymetrix® GeneTitan™ running Affymetrix GeneChip® Command Console 2.0.
|
| Scan protocol |
Standard procedure for Affymetrix® GeneTitan™ running Affymetrix GeneChip® Command Console 2.0.
|
| Description |
wbl20381-040
|
| Data processing |
Scans were imported into ArrayStudio V8.0 and pre-processed using the default RMA pipeline
|
| |
|
| Submission date |
Jun 09, 2015 |
| Last update date |
Dec 09, 2016 |
| Contact name |
Jeannette Bigler |
| E-mail(s) |
jeannette.bigler@bjgroupllc.com
|
| Organization name |
BJ Group, LLC
|
| Street address |
3200 NE 92nd Street
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98115 |
| Country |
USA |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE69683 |
Expression profiling in blood from subjects with severe asthma, moderate asthma, and non-asthmatics collected in the U-BIOPRED study |
|
