|
| Status |
Public on Jul 14, 2015 |
| Title |
AL_15_1 |
| Sample type |
SRA |
| |
|
| Source name |
hippocampal CA1 region
|
| Organism |
Mus musculus |
| Characteristics |
tissue: hippocampal CA1 region
age: 15 months
diet: normal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from frozen hippocampal CA1 microdissections using the miRNAeasy Micro Kit.
300 ng of total RNA with an average RIN value of 9 was applied to the TruSeq RNA Sample Preparation Kit v2 to construct mRNA sequencing libraries.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
020-C
|
| Data processing |
Base calling and demultiplexing was performed using Illumina bcl2fastq software.
The sequencing reads were aligned to the mouse genome (UCSC build mm10) using the TopHat aligner.
Likely PCR duplicates were removed with Picard MarkDuplicates tool.
Mapped reads were analyzed using the Cufflinks package (using fragment bias and multi-read correction).
Genome_build: mm10
Supplementary_files_format_and_content: CSV file contains FPKM values for each sample.
|
| |
|
| Submission date |
Jun 17, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Stephen D Ginsberg |
| E-mail(s) |
ginsberg@nki.rfmh.org
|
| Organization name |
Nathan Kline Institute
|
| Department |
Center for Dementia Research
|
| Street address |
140 Old Orangeburg Road
|
| City |
Orangeburg |
| State/province |
NY |
| ZIP/Postal code |
10962 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE69952 |
Calorie restriction suppresses age-dependent hippocampal transcriptional signatures. |
|
| Relations |
| BioSample |
SAMN03779134 |
| SRA |
SRX1062991 |
