|
| Status |
Public on Mar 31, 2016 |
| Title |
1293 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
group: P10
strain: C57BL/6
|
| Treatment protocol |
Untreated 9 month old livers from male mice carrying different genotypes. P10: Pten deleted in the liver; DM: Pten and Akt2 double deleted livers; WT: controls.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
5125380029_A
Replicate 1
|
| Data processing |
The data were normalized using average normalization
|
| |
|
| Submission date |
Jul 03, 2015 |
| Last update date |
Mar 31, 2016 |
| Contact name |
Penn State COM |
| Organization name |
Penn State College of Medicine
|
| Department |
Genome Sciences Facility
|
| Street address |
500 University Drive
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platform ID |
GPL8389 |
| Series (1) |
| GSE70501 |
Expansion of hepatic tumor progenito cell population in PTEN deficient mice requires liver injury and is reversed by deletion of Akt2 |
|
