|
| Status |
Public on Jul 20, 2015 |
| Title |
2-25-2014, Mouse #929 SAMPLE 127 |
| Sample type |
RNA |
| |
|
| Source name |
PDX mouse
|
| Organism |
Homo sapiens |
| Characteristics |
model: Model HCI-001
cell type: Primary tumor cell
|
| Treatment protocol |
n/a
|
| Growth protocol |
Patient-derived xenograft model
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Enzymatic tissue digest
|
| Label |
SYBR Green
|
| Label protocol |
Single-cell gene expression using SsoFast EvaGreen SuperMix with low Rox on the BioMark system, advanced protocol 41
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Primary tumor cell
|
| Data processing |
Data analyzed using Fluidigm real-time PCR analysis software and custom algorithms in R statistical software
|
| |
|
| Submission date |
Jul 06, 2015 |
| Last update date |
Jul 20, 2015 |
| Contact name |
Devon Lawson |
| E-mail(s) |
Devon.Lawson@ucsf.edu
|
| Organization name |
UCSF
|
| Department |
Anatomy
|
| Lab |
Zena Werb
|
| Street address |
513 Parnassus Ave Hsw 1301
|
| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL20665 |
| Series (2) |
| GSE70552 |
Single-cell analysis reveals a stem cell program in human metastatic breast cancer cells (PDX mice - cancer cells) |
| GSE70555 |
Single-cell analysis reveals a stem cell program in human metastatic breast cancer cells |
|
