|
| Status |
Public on May 12, 2008 |
| Title |
CHD Patient 1562 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
DNA isolated from blood
|
| Organism |
Homo sapiens |
| Characteristics |
DNA isolated from blood of a patient with congenital heart disease (Aortic coarctation). Gender: male
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from peripheral blood by a salting out procedure (Grimberg et al.,1989).
|
| Label |
Cy3
|
| Label protocol |
Probes were labelled by random priming. Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| |
|
| Channel 2 |
| Source name |
DNA isolated from blood
|
| Organism |
Homo sapiens |
| Characteristics |
DNA isolated from blood of a healthy male donor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from peripheral blood by a salting out procedure (Blin and Stafford,1976).
|
| Label |
Cy5
|
| Label protocol |
Probes were labelled by random priming. Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| |
|
| |
| Hybridization protocol |
Hybridization was done overnight at 42°C in a slide booster (Advalytix, Brunnthal, Germany). Detailed protocols are available at our website (Molecular Cytogenetics at the Max Planck Institute for Molecular Genetics)
|
| Scan protocol |
Slides were scanned using an Agilent scanner .
|
| Description |
Array CGH analysis of a patient with congenital heart disease
|
| Data processing |
TIFF images were analysed by Genepix 5.0 (Axon Instruments, Union City, CA) and raw intensities (gpr files) were imported into CGHPRO (Chen at al.,2005). Background intensities were not substracted. Signal intensities were normalized by subgridd LOWESS. Aberrant ratios involving three or more neighbouring clones were considered as genomic aberrations, unless they coincided with a known polymorphism as listed in the Database of Genomic Variants (version Dec 2005)
|
| |
|
| Submission date |
Apr 16, 2007 |
| Last update date |
May 12, 2008 |
| Contact name |
Fikret Erdogan |
| E-mail(s) |
erdogan@molgen.mpg.de
|
| Organization name |
Max-Planck-Institute
|
| Street address |
Ihnestr. 73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL5000 |
| Series (1) |
| GSE7527 |
Array CGH in congenital heart disease |
|
