|
| Status |
Public on Jun 29, 2016 |
| Title |
Macrophage_M2_Lmp7ko_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Alveolar macrophages, M2 population, derived from Lmp7 k.o. mice
|
| Organism |
Mus musculus |
| Characteristics |
age: 8-12 weeks
strain: C57Bl/6J
cell type: Alveolar macrophage
cell population: M2
|
| Treatment protocol |
As described in the paper. Briefly, primary alveolar macrophages were isolated from the lungs of mice by bronchoalveolar lavage (BAL) with 8 washes of 1 ml PBS at room temperature. Polarization towards the M2 phenotype was done with IL-4 (20 ng/ml, Immunotool) treatment for up to 24 h. Unpolarized cells (M0) served as controls.
|
| Growth protocol |
Animals housed under spf conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen) including DNase treatment
|
| Label |
Biotin
|
| Label protocol |
Ovation PicoSL WTA System V2 in combination with the Encore Biotin IL Module (Nugen)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol including minor modification as suggested by Nugen
|
| Scan protocol |
Standard Illumina scanning protocol (HiScan)
|
| Description |
Gene expression data from alveolar macrophages, M2 population, derived from Lmp7 k.o. mice
|
| Data processing |
Data including background subtraction (GenomeStudioV2011.1 software, gene expression module version 1.9.0), removal of negative values by introducing an offset, quantile normalisation
|
| |
|
| Submission date |
Aug 12, 2015 |
| Last update date |
Jun 29, 2016 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE72000 |
Immunoproteasome dysfunction augments alternative polarization of alveolar macrophages |
|
