|
Status |
Public on Apr 06, 2016 |
Title |
48h control |
Sample type |
SRA |
|
|
Source name |
Uninfected larvae
|
Organism |
Plutella xylostella |
Characteristics |
agent: none time point: 48h
|
Treatment protocol |
Third instar P. xylostella larvae were collected into Petri dishes as described above. At first, the sample of 48 hptI and its control were sprayed with 1 ml conidial suspension and 0.02% Tween 80, respectively. After 12 hours, the sample of 36 hptI and its control were sprayed with 1 ml conidial suspension and 0.02% Tween 80. In the same way the sample of 24 hptI and its control was handled. After 48 h of the first spray, approximately 200 living P. xylostella larvae were collected from each of the 6 treatments, respectively. All P. xylostella larvae were collected at the same time can eliminate the effect of host development on gene expression
|
Growth protocol |
P. xylostella culture was maintained on cabbage plants in climate chambers at 25 ± 1 °C, 14 h of light and 10 h of darkness and 75 ± 10% relative humidity; The fungal strain B. bassiana ARSEF2860 from the RW Holley Center for Agriculture and Health (Ithaca, NY, USA) was preserved at 4°C on slants of Sabouraud dextrose agar (Qiu et al., 2014). Conidia harvested from cultures grown for 7 days at 25°C and 90% relative humidity were suspended in 0.02% Tween 80 (109 conidia/ml)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from the P. xylostella using the RNAiso-Plus Reagent (TaKaRa). The cDNA libraries were prepared according to the manufacturer’s instructions (Illumina, San Diego, CA, USA) RNA libraries were prepared for sequencing using standard Illumina protocols
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Illumina HiSeqTM 2000 software used for basecalling. Through quality test we remove low quality sequences, tag with N ratio>5%, empty reads and adaptor sequences then we got clean reads. Map the clean reads to the genome database of P. xylostella and B. bassiana by SOAPaligner/SOAP2 The data was normalized by reads per kilobase transcriptome per million mapped reads (RPKM) genome build: Plutella xylostella: DBM_FJ_V1.1 and Beauveria bassiana: ASM28067v1. Supplementary_files_format_and_content: Table S1-S6 List of detected P. xylostella genes .xlsx Supplementary_files_format_and_content: Table S10-S14. List of DEGs of P. xylostella genes.xlsx Supplementary_files_format_and_content: Table S15-S16 List of different expressed genes of B. bassiana genes.xlsx Supplementary_files_format_and_content: Table S7-S9 List of dected B.bassiana genes.xlsx
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|
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Submission date |
Aug 26, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Chu ZhenJian |
E-mail(s) |
11407006@zju.edu.cn
|
Phone |
15057131676
|
Organization name |
Zhejiang University
|
Department |
College of Life Science
|
Lab |
Institute of Microbiology
|
Street address |
Yuhangtang Road 388
|
City |
Hangzhou |
State/province |
Zhejiang |
ZIP/Postal code |
310058 |
Country |
China |
|
|
Platform ID |
GPL17076 |
Series (1) |
GSE72383 |
New insight into a host-pathogen interaction unveiled by transcriptomics response of the global insect pest Plutella xylostella to a fungal pathogen |
|
Relations |
BioSample |
SAMN04013705 |
SRA |
SRX1165824 |