|
| Status |
Public on Sep 24, 2016 |
| Title |
Control_DLD1_Set1 |
| Sample type |
RNA |
| |
|
| Source name |
DLD1 cells not treated with siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DLD1
cell type: colorectal cancer
|
| Treatment protocol |
siRNA treatment using oligonucleotides against Lamin A/C and Lamin B2 for a duration of 48 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol method
|
| Label |
Cy3
|
| Label protocol |
T7 promoter based-linear amplification to generate labeled complementary RNA (One-Color Microarray-Based Gene Expression Analysis)
|
| |
|
| Hybridization protocol |
Agilent’s In situ Hybridzation kit 5188-5242
|
| Scan protocol |
Not provided
|
| Data processing |
Data Agilent Feature Extraction Software version 10.5 (Protocol GE1_107_Sep09). Normalization Used 50th Percentile shift Normalization
|
| |
|
| Submission date |
Sep 21, 2015 |
| Last update date |
Sep 24, 2016 |
| Contact name |
Kundan Sengupta |
| E-mail(s) |
kunsen@iiserpune.ac.in
|
| Phone |
91-20-25908071
|
| Organization name |
Indian Institute of Science Education and Research (IISER) Pune
|
| Department |
Biology
|
| Lab |
Chromosome Biology Lab
|
| Street address |
Dr. Homi Bhabha Road, Pashan
|
| City |
Pune |
| State/province |
Maharashtra |
| ZIP/Postal code |
411008 |
| Country |
India |
| |
|
| Platform ID |
GPL13252 |
| Series (1) |
| GSE73269 |
Gene expression changes upon Lamin A and Lamin B2 depletion in a diploid colorectal cancer cell line - DLD1 |
|
