|
| Status |
Public on Dec 16, 2015 |
| Title |
PGC-1β(i)skm-/- |
| Sample type |
RNA |
| |
|
| Source name |
PGC-1β(i)skm-/- mutant
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: PGC-1β(i)skm-/-
tissue: gastrocnemius
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA targets were prepared from 150 ng of total RNA using Ambion WT Expression Kit (Cat # 4411974) and Affymetrix GeneChip® WT Terminal Labeling Kit (Cat # 900671) according to Affymetrix recommendations
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.9 μg of cDNAs were hybridized for 16 hours at 45oC, 60 rpm on GeneChip® Mouse Gene 1.0 ST arrays . The chips were then washed and stained in the GeneChip® Fluidics Station 450.
|
| Scan protocol |
The chips were scanned with the GeneChip® Scanner 3000 7G. Raw data were extracted from the scanned images using the Affymetrix GeneChip® Command Console (AGCC) version 4.0
|
| Data processing |
CEL files were processed to calculate probe set signal intensities using Robust Multi-array Average (RMA) algorithms
|
| |
|
| Submission date |
Sep 29, 2015 |
| Last update date |
Dec 16, 2015 |
| Contact name |
Doulaye Dembele |
| E-mail(s) |
doulaye@igbmc.fr
|
| Phone |
+33 3 88 65 35 28
|
| Organization name |
IGBMC
|
| Department |
Biopuces
|
| Street address |
1 rue Laurent Fries
|
| City |
Illkirch |
| ZIP/Postal code |
67400 |
| Country |
France |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE73572 |
The transcriptional coregulator PGC-1β controls mitochondrial function and anti-oxidant defense in skeletal muscles |
|
