|
| Status |
Public on Oct 02, 2015 |
| Title |
FLS_Ad-Epas1_#3 |
| Sample type |
RNA |
| |
|
| Source name |
FLS, Ad-Epas1 infected
|
| Organism |
Mus musculus |
| Characteristics |
passage: Passage 4~8
tissue: Knee synovium
strain: C57BL6
|
| Treatment protocol |
FLS were infected with empty adenovirus or Ad-Epas1 at a multiplicity of infection (MOI) of 800 for 2 h and incubated for 24 hr on 35-mm culture dishes containing 1 ml of RPMI-1640 medium.
|
| Growth protocol |
Murine synovial tissues were minced and digested in collagenase for 4 h at 37℃. The fibroblast-like synoviocytes (FLS) were plated on coverslips in RPMI-1640 medium and incubated for 4 d. FLS between passage 4 and 8 were used for further analysis. Pure FLS (>90% CD90+/<1% CD14+) were identified by flow cytometry using antibodies against the fibroblast marker CD90 and the macrophage marker CD14 (Abcam).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (GeneChip® WT Terminal Labeling and Controls Kit P/N 901525 1, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, fragmented cDNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Mouse Gene 2.0 ST Array. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from murin fibroblast-like synoviocytes infected with Epas1 adenovirus
|
| Data processing |
The Robust Multiarray Average (RMA) algorithm implemented in Affymetrix Expression Console software (version1.3.1) was used to normalize the raw data. Highly expressed genes that showed over 2-fold difference between the signal values of control and test samples were selected for further study.
|
| |
|
| Submission date |
Oct 01, 2015 |
| Last update date |
Oct 02, 2015 |
| Contact name |
Jang-Soo Chun |
| E-mail(s) |
jschun@gist.ac.kr
|
| Organization name |
Gwangju institute of science and technology
|
| Department |
School of Life Science
|
| Lab |
Arthritis Research Lab
|
| Street address |
123 Cheomdan-gwagiro(Oryong-dong), Buk-gu
|
| City |
Gwangju |
| ZIP/Postal code |
61005 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE73658 |
Murine fibroblast-like synoviocytes: Control vs Ad-Epas1 infected |
|
