|
| Status |
Public on Dec 21, 2007 |
| Title |
Rat070216_GENonIMCD_1 |
| Sample type |
RNA |
| |
|
| Source name |
Inner medulla non collecting duct cells of rat
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain: Sprague-Dawley
Sex: Male
Age: 10 weeks old
Tissue: Inner medulla, non collecting duct cells
|
| Treatment protocol |
Rats were injected with 1mg furosemide intraperitoneum 30 minutes before decapitation. Inner Medulla were excised and digested with collagenase B and hyarulonidase for 75 minutes at 37 degree celcius.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted by using Trizol reagent according to manufacturer's protocols.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocols.
|
| |
|
| Hybridization protocol |
Labeled cRNA were hybridized to Rat 230 2.0 Array in Affymetrix Hybridization Oven 640 and washed in GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
|
| Scan protocol |
Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols
|
| Description |
profiling of gene expression in inner medulla collecting duct cell of rat
|
| Data processing |
Affymetrix default parameters
|
| |
|
| Submission date |
May 22, 2007 |
| Last update date |
Dec 21, 2007 |
| Contact name |
Panapat Uawithya |
| E-mail(s) |
uawithyap@nhlbi.nih.gov
|
| Organization name |
NIH
|
| Department |
NHLBI
|
| Lab |
LKEM
|
| Street address |
9000 Rockville pike Bldg 10 Rm 6N306
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE7891 |
Trancriptome profiling of rat inner medullary collecting duct |
|
