|
| Status |
Public on Dec 16, 2015 |
| Title |
2010 HIPC TIV subject ID 60 at D14 post-vaccination |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood mononuclear cells of subjects vaccinated with Influenza vaccine at day 0 (before vaccination), and days 3 and 7 post-vaccination.
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: mononuclear cells
time point: D14
subject id: 60
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Following PMBC isolation from CPT, 2 x 10^6 cells were lysed in 1 ml of TRIzol and stored at -80C (Cat# 15596-026; Invitrogen Life Technologies). After all time points were collected for a subject, the samples were thawed, and the RNA isolation proceeded according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Total RNA sample quality was evaluated by spectrophotometer to determine quantity, protein contamination and organic solvent contamination, and an Agilent 2100 Bioanalyzer was used to check for RNA degradation. Two-round in vitro transcription amplification and labeling was performed starting with 50 ng intact, total RNA per sample, following the Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization was performed on Human U133 Plus 2.0 Arrays (using GeneTitan platform, Affymetrix, or individual cartridges) for 16 h at 45 oC, and 60 r.p.m. in a Hybridization Oven 640 (Affymetrix), slides were washed and stained with a Fluidics Station 450 (Affymetrix).
|
| Scan protocol |
Scanning was performed on a 7th generation GeneChip Scanner 3000, and the Affymetrix software was used to perform image analysis and generate raw intensity data.
|
| Data processing |
RMA normalization was performed using Expression Console software.
|
| |
|
| Submission date |
Nov 09, 2015 |
| Last update date |
Feb 25, 2021 |
| Contact name |
Helder I. Nakaya |
| E-mail(s) |
hnakaya@usp.br
|
| Phone |
+551126481130
|
| Organization name |
Universidade de São Paulo
|
| Lab |
csbiology.com
|
| Street address |
AVENIDA PROFESSOR LINEU PRESTES, 580, Block 17
|
| City |
SÃO PAULO |
| State/province |
SÃO PAULO |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL13158 |
| Series (2) |
| GSE74813 |
Time Course of Adults Vaccinated with Influenza TIV Vaccine during 2010/11 Flu Season (HIPC cohort) |
| GSE74817 |
Time Course of Adults Vaccinated with Influenza TIV Vaccine 2009-2012 |
|
