|
| Status |
Public on Nov 12, 2015 |
| Title |
T48 RPH_Rat1_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
untreated RPHs_48hrs post-seeding
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
rat id: Rat1
gender: male
tissue/cell type: rat primary hepatocytes (RPH)
time in culture: 48hrs post-seeding
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were homogenized in Trizol, and RNA isolated per manufacturer's protocol. Impurities were removed from the resulting RNA using Qiagen RNeasy columns.
|
| Label |
biotin
|
| Label protocol |
RNA was then labeled and hybridized to RG230-2 microarrays (Affymetrix) according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Affymetrix hyb protocol: EukGE-WS2v5_450
|
| Scan protocol |
.CEL files generated using GCOS software
|
| Description |
RPH_T48-2
|
| Data processing |
MAS5 signal intensities were generated using Bioconductor's Affy package. Data was scaled to a target of 500.
|
| |
|
| Submission date |
Nov 11, 2015 |
| Last update date |
Nov 12, 2015 |
| Contact name |
Jeffrey Sutherland |
| E-mail(s) |
sutherlandje@lilly.com
|
| Organization name |
Lilly Research Laboratories
|
| Street address |
Lilly Corporate Center
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46285 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE74903 |
Assessing concordance of drug-induced transcriptional response in rodent liver and cultured hepatocytes |
|
