|
Status |
Public on Nov 28, 2016 |
Title |
CRC Patient 156 |
Sample type |
RNA |
|
|
Source name |
primary colon adenocarcinoma
|
Organism |
Homo sapiens |
Characteristics |
patient id: 156 analysis batch: 1 other geo code: #N/A! age at diagnosis: 68 gender: female tumor stage: Tumor stage III primary site: right microsatellite status: MSI-H braf v600e status: wt kras codon 12, 13, 61 status: wt
|
Extracted molecule |
total RNA |
Extraction protocol |
Surgically isolated proximal colon cancer specimens were immediately frozen in liquid nitrogen. Total RNA was extracted from cancer tissue using the AllPrep DNA/RNA Micro Kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Approximately 8 ug of total RNA was processed to produce biotinylated cRNA targets.
|
|
|
Hybridization protocol |
Standard Affymetrix procedures
|
Scan protocol |
Standard Affymetrix procedures
|
Data processing |
RMA-sketch normalization on the core probeset using Affymetrix Power Tools (APT) followed by batch correction using the ComBat algorithm.
|
|
|
Submission date |
Nov 23, 2015 |
Last update date |
Nov 28, 2016 |
Contact name |
Oliver Sieber |
Organization name |
Walter and Eliza Hall Insitute of Medical Reseach
|
Street address |
1G Royal Parade
|
City |
Parkville |
State/province |
VIC |
ZIP/Postal code |
3052 |
Country |
Australia |
|
|
Platform ID |
GPL5175 |
Series (2) |
GSE75315 |
Expression data for 211 primary tumors from patients with colorectal cancer |
GSE75317 |
BRAF V600E mutant colorectal cancer subtypes based on gene expression |
|