|
| Status |
Public on Nov 28, 2016 |
| Title |
CRC Patient 162 |
| Sample type |
RNA |
| |
|
| Source name |
primary colon adenocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
patient id: 162
analysis batch: 1
other geo code: GSM1554076
age at diagnosis: 80
gender: male
tumor stage: Tumor stage III
primary site: right
microsatellite status: MSS
braf v600e status: wt
kras codon 12, 13, 61 status: mut
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Surgically isolated proximal colon cancer specimens were immediately frozen in liquid nitrogen. Total RNA was extracted from cancer tissue using the AllPrep DNA/RNA Micro Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Approximately 8 ug of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Data processing |
RMA-sketch normalization on the core probeset using Affymetrix Power Tools (APT) followed by batch correction using the ComBat algorithm.
|
| |
|
| Submission date |
Nov 23, 2015 |
| Last update date |
Nov 28, 2016 |
| Contact name |
Oliver Sieber |
| Organization name |
Walter and Eliza Hall Insitute of Medical Reseach
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| State/province |
VIC |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL5175 |
| Series (2) |
| GSE75315 |
Expression data for 211 primary tumors from patients with colorectal cancer |
| GSE75317 |
BRAF V600E mutant colorectal cancer subtypes based on gene expression |
|
