|
Status |
Public on Nov 02, 2016 |
Title |
EpOC from UC patient [154S] |
Sample type |
RNA |
|
|
Source name |
EpOC from biopsy sample of UC patient
|
Organism |
Homo sapiens |
Characteristics |
case_id: 154 case_phenotype: ulcerative colitis (UC) patient organoid_culture: EpOC tissue: sigmoid colon endoscopic_mayo_score: 1 uc_evolution_time (years): 8 age (years): 41
|
Growth protocol |
Crypts from descending/sigmoid colon samples were isolated by EDTA treatment. Crypt units were embedded in Matrigel, expanded for 3-4 days in "stem medium" and dissociated. Single epithelial cells were replated in fresh "stem medium" according to a 1:3 dilution. At day 5 of in vitro expansion, total RNA was isolated from the EpOCs. Part of the EpOCs was induced to differentiate (d-EpOCs) by the addition of fresh "differentiation medium". Total RNA was isolated from d-EpOCs 5 days after differentiation.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Trizol+RNA Cleanup protocol by RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
|
|
Hybridization protocol |
Standard Affymetrix protocol
|
Scan protocol |
Standard Affymetrix protocol using a Gene chip scanner 3000
|
Description |
Gene expression data of EpOC; in vitro organoid culture derived from biopsy sample of UC patient.
|
Data processing |
The data were analyzed with Bioconductor tools in R (version 3.1.0; http://www.r-project.org) using GC-RMA as normalization method. Next we employ a probe-filtering step, excluding those probes that showed a lower coefficient of variation or which were non-informative (not annotated).
|
|
|
Submission date |
Dec 11, 2015 |
Last update date |
Nov 02, 2016 |
Contact name |
Azucena Salas |
E-mail(s) |
asalas1@recerca.clinic.cat
|
Organization name |
IDIBAPS
|
Department |
Gastroenterology
|
Street address |
Rosselló, 149-153
|
City |
Barcelona |
ZIP/Postal code |
08036 |
Country |
Spain |
|
|
Platform ID |
GPL20650 |
Series (1) |
GSE75916 |
Expression data of epithelial organoid cultures generated from intestinal mucosa of non-IBD controls and patients with ulcerative colitis |
|