|
| Status |
Public on Dec 16, 2015 |
| Title |
1_124001 |
| Sample type |
RNA |
| |
|
| Source name |
primary breast cancer from post menopausal patients that developed metastatic disease
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: primary breast cancer
age: 58
er (allred score): 4
pr (allred score): 2.5
ki67 (%): 0
her2: 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from FFPE tumor samples using Rneasy FFPE kits ( Qiagen) and amplified with WT-Ovation FFPE Sytem V2 ( NuGEN).
|
| Label |
biotin
|
| Label protocol |
CDNA was labeled using FL-Ovation cDNA Biotin Module V2 ( Nugen).
|
| |
|
| Hybridization protocol |
Samples were run on the Affymetrix Human Transcriptome Array ( HTA) 2.0.
|
| Scan protocol |
An Affymetrix Fluidics station 450 and Affymetrix GeneChip Scanner 3000 7G were used per manufactures' instructions.
|
| Data processing |
QC and normalization with RMA was conducted using Affymetrix expression console.
|
| |
|
| Submission date |
Dec 15, 2015 |
| Last update date |
Dec 16, 2015 |
| Contact name |
Rinath Jeselsohn |
| Organization name |
DFCI
|
| Department |
Medical Oncology
|
| Lab |
Jeselsohn Lab
|
| Street address |
450 Brookline Ave, D728
|
| City |
Boston |
| State/province |
MD |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE76040 |
transCONFIRM gene expression analysis |
|
