|
Status |
Public on Sep 30, 2016 |
Title |
bronchial biopsy_moderate_rep15 |
Sample type |
RNA |
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|
Source name |
bronchial biopsy of moderate asthma
|
Organism |
Homo sapiens |
Characteristics |
tissue: lung tissue source: bronchial biopsy diagnosis: moderate asthma gender: male age: 45 oral corticosteroid use: no
|
Extracted molecule |
total RNA |
Extraction protocol |
column-based extraction of total RNA was performed according to the manufacturer's instructions
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip Expression Analysis Technical Manual, Copyright 2005-2009, Affymetrix)
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|
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneChip Scanner 3000.
|
Description |
bronchial biopsy of moderate asthma
|
Data processing |
The data were quality controlled and pre-processed using R code of Affymetric Analysis QC workflow from ArrayAnalysis.org with Robust Multichip Average (RMA) as normalization method.
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|
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Submission date |
Dec 21, 2015 |
Last update date |
Sep 30, 2016 |
Contact name |
Chih-hsi Scott Kuo |
E-mail(s) |
Chihhsikuo@gmail.com
|
Organization name |
Imperial College London
|
Department |
Department of Computing
|
Street address |
South Kensington Campus
|
City |
London |
ZIP/Postal code |
SW7 2AZ |
Country |
United Kingdom |
|
|
Platform ID |
GPL13158 |
Series (2) |
GSE76225 |
Expression data of bronchial biopsies from Unbiased BIOmarkers in Prediction of REspiratory Disease outcomes (U-BIOPRED) Project |
GSE76227 |
Expression data of bronchial biopsies and epithelial brushing from Unbiased BIOmarkers in Prediction of REspiratory Disease outcomes (U-BIOPRED) Project |
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