|
| Status |
Public on Sep 30, 2016 |
| Title |
bronchial biopsy_severe_rep24 |
| Sample type |
RNA |
| |
|
| Source name |
bronchial biopsy of severe asthma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung
tissue source: bronchial biopsy
diagnosis: severe asthma
gender: female
age: 58
oral corticosteroid use: yes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
column-based extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (GeneChip Expression Analysis Technical Manual, Copyright 2005-2009, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneChip Scanner 3000.
|
| Description |
bronchial biopsy of severe asthma
|
| Data processing |
The data were quality controlled and pre-processed using R code of Affymetric Analysis QC workflow from ArrayAnalysis.org with Robust Multichip Average (RMA) as normalization method.
|
| |
|
| Submission date |
Dec 21, 2015 |
| Last update date |
Sep 30, 2016 |
| Contact name |
Chih-hsi Scott Kuo |
| E-mail(s) |
Chihhsikuo@gmail.com
|
| Organization name |
Imperial College London
|
| Department |
Department of Computing
|
| Street address |
South Kensington Campus
|
| City |
London |
| ZIP/Postal code |
SW7 2AZ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13158 |
| Series (2) |
| GSE76225 |
Expression data of bronchial biopsies from Unbiased BIOmarkers in Prediction of REspiratory Disease outcomes (U-BIOPRED) Project |
| GSE76227 |
Expression data of bronchial biopsies and epithelial brushing from Unbiased BIOmarkers in Prediction of REspiratory Disease outcomes (U-BIOPRED) Project |
|
