|
| Status |
Public on Jun 27, 2017 |
| Title |
CCA Non-Tumor Tissue LCS_631B |
| Sample type |
RNA |
| |
|
| Source name |
CCA Non-Tumor Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Liver Non-Tumor Tissue
disease state: Cholangiocarcinoma (CCA)
individual: LCS_631
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen tissues using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol. RNA integrity for each sample was confirmed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures using GeneChip Scanner 3000 and AGCC software
|
| Data processing |
Raw gene expression data were analyzed on gene level and normalized using the Robust Multi-array Average (RMA) method and sketch-quantile normalization method on Affymetrix Expression Console Software Build 1.3.1.187.
|
| |
|
| Submission date |
Dec 23, 2015 |
| Last update date |
Jun 27, 2017 |
| Contact name |
Xin Wei Wang |
| E-mail(s) |
xw3u@nih.gov
|
| Phone |
240-760-6858
|
| Organization name |
National Cancer Institute
|
| Department |
Laboratory of Human Carcinogenesis
|
| Lab |
Liver Carcinogenesis Unit
|
| Street address |
37 Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-4255 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (2) |
| GSE76297 |
Gene expression data of human hepatocellular carcinoma (HCC) and Cholangiocarcinoma (CCA) from Thailand Initiative in Genomics and Expression Research for Liver Cancer (TIGER-LC) |
| GSE76311 |
Human hepatocellular carcinoma (HCC) and Cholangiocarcinoma (CCA) from Thailand Initiative in Genomics and Expression Research for Liver Cancer (TIGER-LC) |
|
