|
| Status |
Public on May 03, 2016 |
| Title |
Patient 25 Normal |
| Sample type |
SRA |
| |
|
| Source name |
Liver
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver
diagnosis: Normal
|
| Treatment protocol |
All samples were stored at -80ºC prior to processing.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were extracted from snap-frozen tissue sections using Trizol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Data processing |
quality control
mapping with Tophat2
quantify with Cufflinks
normalization
differential gene analysis with Cufflinks
Genome_build: hg19
Supplementary_files_format_and_content: normalized expression level of samples, diffrentially expressed genes between normal and tumor
|
| |
|
| Submission date |
Jan 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Gang Liu |
| E-mail(s) |
gangliu@sibs.ac.cn
|
| Phone |
86-021-54920084
|
| Organization name |
Shanghai Institutes for Biological Sciences
|
| Street address |
320 Yueyang Rd, Shanghai
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL9052 |
| Series (1) |
| GSE77314 |
RNA-seq of 50 paired hepatocellular carcinoma |
|
| Relations |
| BioSample |
SAMN04445041 |
| SRA |
SRX1550007 |
