|
| Status |
Public on Sep 01, 2016 |
| Title |
Astrocyte_non IgG_LPS(+)_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Human astrocyte exposure to non IgG with LPS (1μg/ml) for 24 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: astrocytes
treatment: non IgG with LPS (1μg/ml) for 24 h
|
| Growth protocol |
Normal human astrocytes (NHAs) isolated from human fetal brains (Clonetics normal human astrocytes; Lonza, Switzerland) were maintained in RPMI 1640 supplemented with 10 % fetal calf serum and seeded into 6-well tissue culture plates to a density of 4 × 10^5 cells 24 hours prior to experimentation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit (Qiagen, Miami, FL) was used.
|
| Label |
Cy5
|
| Label protocol |
We followed manufacturer's protocol. (http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf)
|
| |
|
| Hybridization protocol |
We followed manufacturer's protocol. (http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf)
|
| Scan protocol |
We followed manufacturer's protocol, and used GenePix 4400A. (http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf)
|
| Data processing |
CodeLink Expression Analysis v5.0 software, Quantile normalization
|
| |
|
| Submission date |
Mar 14, 2016 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Kunihiro Ichinose |
| E-mail(s) |
kichinose@med.shimane-u.ac.jp
|
| Organization name |
Shimane University Faculty of Medicine
|
| Department |
Department of Rheumatology
|
| Street address |
89-1 Enya-cho
|
| City |
Izumo |
| ZIP/Postal code |
693-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE79189 |
The gene expression analysis of human astrocyte exposure to anti-suprabasin antibody: identification of a novel antibody for neuropsychiatric systemic lupus erythematosus |
|
