|
| Status |
Public on Mar 22, 2016 |
| Title |
SKOV3_CFG_monomer_TGFbeta-2 |
| Sample type |
RNA |
| |
|
| Source name |
human ovarian cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
cell line: SKOV3
|
| Treatment protocol |
After serum starvation for 24 h, the ovarian cancer cells were treated with 3 mg/ml CFG only or in combination with 10 ng/ml TGFβ1 for 24 h
|
| Growth protocol |
maintained in DMEM supplemented with 10% fetal bovine serum at 37℃ in a humidified 5% CO2 atmosphere.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from treated and non-treated cell lines using Trizol reagent (Invitrogen, Carlsbad, San Diego, USA) following the manufacturer’s protocol
|
| Label |
biotin
|
| Label protocol |
The amplified RNA was prepared with the GeneChip 3’IVT Express Kit (Affymetrix Inc., Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
After fragmentation, the amplified transcripts were hybridized to an Affymetrix 901838 GeneChip PrimeView Human Gene Expression Array (Affymetrix Inc.) for 16 h in a GeneChip Hybridization Oven 645
|
| Scan protocol |
Arrays were washed and stained by a GeneChip Fluidics Station 450 and scanned by a GeneChip Scanner 3000
|
| Data processing |
Raw data were imported into GeneSpring GX 11.0.1 and analyzed
|
| |
|
| Submission date |
Mar 21, 2016 |
| Last update date |
Mar 22, 2016 |
| Contact name |
Fangfang Tao |
| E-mail(s) |
taoff@zcmu.edu.cn
|
| Organization name |
Zhejiang Chinese Medical University
|
| Street address |
540 Binwen Road，binjiang district
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310053 |
| Country |
China |
| |
|
| Platform ID |
GPL16043 |
| Series (1) |
| GSE79454 |
Microarray to identify the differentially expressed genes in CFG treated SKOV3 cells compared with control cells under TGFbeta condition |
|
