 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 06, 2017 |
| Title |
Adult_innate_memory |
| Sample type |
SRA |
| |
|
| Source name |
Single positive CD8+ T cells from the spleen
|
| Organism |
Mus musculus |
| Characteristics |
mouse age: 2-4 months (adult)
tissue: Spleen
isolation protocol: Positive immunomagnetic selection for CD8, followed by FACS sorting for CD44highCD122high cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol from purified cell populations
NEBNext Ultra RNA Library Kit (NEB) was used on 25-200 ng RNA
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Nucleotides with Phred scores <20 were trimmed from read ends
Trimmed reads >18 nt in length were aligned to the genome with Tophat v2.1.1
Aligned reads were quantified and differnetial expression was found using CuffDiff v2.2.1
Genome_build: mm9
Supplementary_files_format_and_content: CuffDiff output file. Columns include gene name, sample 1 name, sample 2 name, test status, sample 1 FPKM value, sample 2 FPKM value, log2 fold-change, test statistic, p value, q value, and if signficant at a FDR of 5%
|
| |
|
| Submission date |
Apr 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrew Grimson |
| Organization name |
Cornell University
|
| Department |
Molecular Biology & Genetics
|
| Street address |
445 Biotechnology Building
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE80597 |
Fetal and adult progenitors give rise to unique populations of CD8+ T cells |
|
| Relations |
| BioSample |
SAMN04889578 |
| SRA |
SRX1722151 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
